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The substrate Alternative was then saved at −20°C until the ammonium and nitrate contents were being analyzed Using the San++ Continual-Stream Analysator (Skalar Analytic GmbH, Erkelenz, Germany). Nitrate perseverance was according to a reaction with hydrazinium sulfate, sulfanilamide and N-(1-naphthyl) ethylenediamine dihydrochloride which ends